The Caspase‐1 inhibitor AC‐YVAD also inhibited both Caspase‐6 activity and GSDMC activation (Figure 5C,D), consistent with previous reports that Caspase‐6 can be activated by Caspase‐1.[26, 27, 28] To further confirm the role of Caspase‐6 in activating GSDMC in mouse colon and CRC tissues, colons isolated from Caspase‐6 knockout (Casp6 KO) mice were cultured ex vivo with OGD treatment, and GSDMC activation was significantly reduced in Casp6 KO colon tissues (Figure 5E). This evidence concerns the gene GSDMC and colorectal carcinoma.