To explore the mechanism of GSDMC in promoting CRC development, we first established CT26 and MC38 mouse colorectal cancer cell lines stably overexpressing GSDMC2–4 or expressing shRNA targeting GSDMC2‐4 (shGSDMC) and confirmed the overexpression or knockdown of GSDMC2‐4 by qPCR and Western blot (Figure S6A,B, Supporting Information). Here, GSDMC is linked to colorectal carcinoma.