In situ tissue flow cytometry analysis demonstrated that in normal tissues adjacent to tumor of the control mice, the proportion of ERα-positive cells among CK8+ cells were 45.49%, whereas this proportion was just 9.28% in MMTV-Cre+; Frmd8fl/fl; PyMT mice (Figure 3—figure supplement 1A). This evidence concerns the gene KRT8 and neoplasm.