Cell Counting Kit-8 assay and quantitative real-time PCR were used to validate the expression level of SLFN11 and drug sensitivity in colorectal cancer samples.<h4>Result</h4>Our study revealed that SLFN11 was downregulated in most cancers and correlated with DNA repair, the P53 pathway and immune response in tumor development progress by multi-omics analysis. Here, TP53 is linked to colorectal cancer.