<h4>Background</h4>Schlafen11 (SLFN11) is a key gene in p53-independent apoptosis through ribosome stalling; however, systematic research has been conducted on its role in the tumor immune microenvironment, clinical application, and immunotherapy response across pan-cancer.<h4>Method</h4>Public data were downloaded and multi-omics approaches were used to investigate the relationship between the expression level of SLFN11 and spatial position, biological function, immune landscape, and clinical application values. This evidence concerns the gene TP53 and neoplasm.