<h4>Background</h4>Schlafen11 (SLFN11) is a key gene in p53-independent apoptosis through ribosome stalling; however, systematic research has been conducted on its role in the tumor immune microenvironment, clinical application, and immunotherapy response across pan-cancer.<h4>Method</h4>Public data were downloaded and multi-omics approaches were used to investigate the relationship between the expression level of SLFN11 and spatial position, biological function, immune landscape, and clinical application values. The gene discussed is SLFN11; the disease is cancer.