Finally, all five genes were confirmed to be decreased in MIN6 cells treated with high glucose and palmitic acid.<h4>Conclusion</h4>SLC2A2, ENTPD3, ARG2, CHL1, and RASGRP1 could be used as the mitochondria-related feature genes to predict type 2 diabetes mellitus and the therapeutic targets. Here, ARG2 is linked to diabetes mellitus.