PDK1 (3-phosphoinositide-dependent protein kinase-1), were identified as possible targets of three miRNAs of let-7 family in our study, which plays an important role in regulating glucose homoeostasis and controlling expression of insulin-regulated genes, and a deficiency of the PDK1 pathway in the liver could contribute to development of diabetes, as well as to liver failure. Here, INS is linked to Hepatic failure.