This study aims to investigate the functional impact of non-synonymous single-nucleotide polymorphisms (nsSNPs) in RAD50 (a breast cancer-associated gene) and focuses on their consequences on protein structure and interaction within the MRN complex.<h4>Methods</h4>A total of 1,806 nsSNPs were retrieved and subjected to variant analysis using a set of computational tools and ConSurf. The gene discussed is RAD50; the disease is breast carcinoma.