Described changes in cancer KC enzyme activities suggested modifications in the reactions that control the KC flux compared to normal cells.<h4>Methods</h4>In this work, kinetic metabolic models of KC of mitochondria from cancer (HepM), liver (RLM) and heart (RHM) to identify targets to decrease the KC flux were constructed from kinetic parameters (Vmax and Km) of enzymes here determined.<h4>Results</h4>The enzymes Vmax values were higher in the following order: RHM > HepM > RLM; meanwhile, Km values were similar. This evidence concerns the gene CALCA and cancer.