C8orf76 and cancer: Experimental validation in LUAD A549 cells demonstrated that the knockdown of C8orf76 significantly inhibited cell proliferation in LUAD.<h4>Conclusion</h4>The present study is the first to report that the multi-omics pan-cancer analysis predicts C8orf76 as a promising target in cancer prognosis, diagnosis, immunology, and chemotherapy, highlighting its influence on cell proliferation in LUAD with experimental validation.