With regards to the TB clinical range, Yuan et al.128 measured 100–106 pg/mL levels,which could potentially monitor and differentiate active TB or latentTB using these higher linear ranges.130 Furthermore, the biosensor proposed by Gao et al.129 had a broad relevant clinical range of 5–5000 pg/mLthat includes the suggested 5 pg/mL cutoff value for TNF-α.In contrast to the pg/mL LODs observed with ECL, the study by Yolaet al.131 demonstrated a 1 fg/mL LOD usingthe alternative electrochemical biosensor. The gene discussed is TNF; the disease is tuberculosis.