Additionally, the knockdown of GART effectively inhibited the cell proliferation and migration of LC cells both <i>in vitro</i> and <i>in vivo.</i> Mechanistically, qRT-PCR and western blot analyses suggested that GART deletion could inhibit the activation of the PAICS-Akt-β-catenin pathway <i>in vivo</i>.<h4>Conclusions</h4>Our study indicated a tumor-promoting function of GART in LC through the regulation of the PAICS-Akt-β-catenin axis, and it may be used as a therapeutic target for NSCLC. Here, PAICS is linked to neoplasm.