VPS18 and hepatocellular carcinoma: VPS11-KS (Fig 3B left) and VPS18-KS (Fig 3B right) parasites had a significant build-up of vesicles in the cytosol, apparent at early trophozoites (18–26 hpi) and this became more pronounced at late trophozoite stages (26–34 h.p.i), suggestive of a defect in trafficking of HCC containing vesicles to the DV.