In vitro experiments are performed by HK2cell line to simulate the normal oxygen state (Normoxia) and the hypoxia state (Hypoxia) caused by heart failure, SIRT1 gene over‐expression by transfected vectors, knock‐down and Rapamycin (RAPA)‐induced cellular autophagy, and the cell models are divided into four subgroups, named control group, oeSIRT1, siSIRT1 and siSIRT1+RAPA. The gene discussed is SIRT1; the disease is heart failure.