<h4>Background</h4>This study seeks to investigate the potential synergistic effects of combining ultrasound-guided percutaneous radiofrequency ablation with anti-PD-1 therapy on prostate cancer, utilizing animal models.<h4>Methods</h4>A mouse model of prostate cancer was established by subcutaneous injection of 1 × 10<sup>6</sup> Myc-Cap cells on the right side of FVB mice. Here, MYC is linked to Familial prostate cancer.