Given orexin's well-documented role in enhancing memory and cognition, this study investigates its potential to regulate Aβ-induced neurotoxicity, offering new perspectives into AD management.<h4>Methods</h4>This paper simulated Aβ accumulation in the hippocampus of AD patients by administering Aβ<sub>1-42</sub> oligomers into the bilateral hippocampal dentate gyrus of ICR mice. This evidence concerns the gene HCRT and Alzheimer disease.