The impact of the PKC inhibitor Gö 6983 and IRS-1 overexpression via adenoviral vectors (AdIRS-1) on the PKC/IRS-1/ERK signaling pathway and PASMCs behavior was analyzed through Western blotting, EdU incorporation assay, and wound healing assay.<h4>Results</h4>In PAH rats, there was a significant rise in mPAP and RVHI (<i>p</i> < 0.05), accompanied by notable pulmonary vascular remodeling. The gene discussed is PRRT2; the disease is pulmonary arterial hypertension.