The objective of this research was to explore the role of the protein kinase C (PKC)/IRS-1/ERK signaling pathway in the progression of PAH and its influence on the proliferation and migration of PASMCs.<h4>Methods</h4>To establish the PAH model, low-dose Monocrotaline (MCT) was intraperitoneally administered to male SD rats twice a week. This evidence concerns the gene IRS1 and pulmonary arterial hypertension.