By repeating the experiments in B16F10 cells, which originally lacked these neoantigens, the cytotoxicity of VT-NF cells in Hepa1-6 cells was reproduced in Adv-NFH-infected but not in PBS- or Adv-Ctrl-infected B16F10 cells, indicating specific recruitment of vaccine-primed CD8+ cells by transferring the neoantigen peptide-MHC complexes to the target tumor cells (Fig. 2G). The gene discussed is NEFH; the disease is neoplasm.