NEFH and carcinoma: Using pre-infected murine carcinoma (LLC and B16F10) and human (HEK293T and HepG2) cell lines as target cells for cytotoxicity assay, the peptide vaccine primed T cells demonstrated similar cytotoxicity against Adv-NFH- and Adv-NGSH-infected target cells, while displaying negligible cytotoxicity toward PBS- or Adv-Ctrl-treated cells (Fig. 2D).