Thus, by demonstrating the utilization of Vac-NF as an effective neoantigen vaccine in preventing tumor establishment of the cells (B16F10) beyond the resident cells of the neoantigens (Hepa1-6), these results raised a possibility to put into a practice of functional integrative therapy that consist of neoantigen vaccination and intratumoral delivery of neoantigen peptide-MHC complexes. This evidence concerns the gene HLA-C and neoplasm.