Consistent with the results of cytotoxicity assay with vaccine-primed CD4+ and CD8+ cells (Fig. 2F,G), in contrast to the ineffectiveness of CD4 depletion, CD8 depletion completely abolished the therapeutic benefits of the integrative therapy in B16F10 established tumor models (Fig. 5G–I), verifying CD8+ T cells as a crucial effector for the therapeutic outcome of the integrative therapy. Here, CD4 is linked to neoplasm.