Flow cytometry analysis of CD4+ Tumor-Infiltrating Lymphocytes (TILs) and draining lymph nodes from B16F10 tumor-bearing mice revealed increased IFNγ production in CD4Nlrp3-/- mice compared with control mice at both the mRNA (Supplementary Fig. 4a) and protein levels (Supplementary Fig. 4b and Fig. 1F). Here, IFNG is linked to neoplasm.