To assess the impact of obesity and HFD affect energy production, lipid metabolism, and uterine contraction through MAMs disruption, we performed TEM, which showed increased distances between the ER and mitochondrial membranes in obesity, HFD‐fed mice, PA‐treated USMCs, and AAV‐FABP4 group indicating disrupted MAMs. The gene discussed is FABP4; the disease is obesity due to melanocortin 4 receptor deficiency.