In addition, mice treated with Re-FMT in combination with αPD-1 also had lower developmental abnormality scores, significantly lower Ki-67 levels in the tumor and non-tumor colon tissues, significantly more P53-positive cells in tumor and non-tumor colon tissues (Figures 2L-N), and significantly increased protein expression of the tight junction protein ZO-1 and the mucin MUC2 (Supplementary Figure 1A, B). This evidence concerns the gene MUC2 and neoplasm.