An elevation in glypican-4 levels was associated with an increased risk of GDM, with a statistically higher risk observed in pregnant women with glypican-4 levels exceeding 0.40 ng/mL in the first trimester (5-12 weeks of gestation) or 0.79 ng/mL in the second trimester (13-23 weeks of gestation). The gene discussed is GPC4; the disease is gestational diabetes.