To further assess the role of FLCN in hepatocarcinogenesis, we induced liver injury and recovery by administering a 25 mg/kg dose of diethylnitrosamine (DEN) in FlcnLiKO and control mice at post-natal stage (P) 21 (Fig. 4A), a well-established protocol for liver cancer induction [28, 31, 32]. The gene discussed is FLCN; the disease is liver cancer.