GCLC and renal cell carcinoma: Human renal cell carcinoma (RCC) cells ACHN were treated with 700 μM TBH, and the results showed that cellular GSH level was decreased at 3 h post treatment, but rapidly increased thereafter (Fig. 2A), suggesting that oxidative stress leads to exhaustion of cellular GSH at earlier treatment which could be rapidly compensated through activated GCLC activity.