We further generated a PPI network of DEPs from IFI30 knockdown in ESCC cells using the STRING database and determined the top 10 hub proteins using Cytoscape software, including CYCS, CALR, MAPK9, SNRPD2, LAMP1, STT3A, HNRNPR, APP, UBE2N, and H2AX (Figure 6a,b). The gene discussed is CALR; the disease is esophageal squamous cell carcinoma.