TNF and hydrops fetalis: To further ascertain the exact cellular source of TNF-α and CXCL2 in inflamed skin, we categorized skin-infiltrated and skin-resident cells into monocytes (CD11b+Ly6G−Ly6C+), neutrophils (CD11b+Ly6G+Ly6C−), DCs (CD11c+MHC-II+), macrophages (CD11b+F4/80+), T cells (CD45+CD3+), HF-keratinocyte (CD45−Sca-1−), inter-HF-keratinocyte (CD45-Sca-1+) and purified them using flow cytometry.