Heatmap analysis of differentially expressed proteins revealed that key ferroptosis-associated proteins—including transferrin (TF), glutamine cysteine ligase catalytic subunit (GCLC), and glutamine cysteine ligase modifier subunit (GCLM)—exhibited consistent expression patterns across TIMP1-modulated cells, primary tumors, and metastatic tumors. This evidence concerns the gene TIMP1 and metastatic neoplasm.