Heatmap analysis of differentially expressed proteins revealed that key ferroptosis-associated proteins—including transferrin (TF), glutamine cysteine ligase catalytic subunit (GCLC), and glutamine cysteine ligase modifier subunit (GCLM)—exhibited consistent expression patterns across TIMP1-modulated cells, primary tumors, and metastatic tumors. The gene discussed is GCLC; the disease is metastatic neoplasm.