SFN (15 μM) treatment for 72 h exhibited substantial cholesterol reduction, whereas SFN treatment for 24 h showed a relatively weaker cholesterol clearance in MEF cells (Figure 5C and D) compared with human NPC1 cells (Figure 1C), suggesting that human NPC cells are more sensitive to SFN treatment compared to mouse NPC cells. Here, NPC1 is linked to nasopharyngeal carcinoma.