To investigate the inhibitory effect of Nav on the inflammatory response to myocardial I/R in mice, we used RT‒PCR to measure the levels of the inflammation-related markers IL-1β, IL-6, CXCL1 and CXCL8 in mouse heart tissue, and the results showed that the expression of these inflammatory factors in the heart tissue of mice treated with Nav was significantly lower than that in the MI group (Figure 4A). This evidence concerns the gene IL1B and myocardial infarction.