When the number of cells containing trogosomes was quantified and compared to the number of cells containing no trogosomes, we discovered that longer co-culture durations led to increased trogosome acquisition by the tumor cells, with a 16-h co-culture resulting in over 60% of TNBC cells acquiring CD45 (Fig. 3B; n = 50 cells per replicate, per time point). This evidence concerns the gene PTPRC and neoplasm.