To detect the effect of PI3K-AKT-mTOR pathway changes on bladder cancer T-24 cell line after compound ChA treatment, the experiment used western blot to detect PI3K, AKT, and mTOR proteins, and selected phosphorylation sites to detect p-PI3K-p58, p-AKT-Ser473, and p-mTOR-Ser2448 expression changes. The gene discussed is AKT1; the disease is urinary bladder cancer.