In this case, the tumor exhibited the classic H3K27M protein expression alongside ATRX loss, moderate TP53 expression, EGFR overexpression by IHC, and H3F3A K28M mutation along with PTEN G132V – subclonal, RAD51B loss on exon 8, TSC2 E1344del, ATRX splice site 6849 + 2T>C by next generation sequencing (Foundation Medicine, Cambridge, MA). Here, TSC2 is linked to neoplasm.