To verify the effects of both PSMD11 and PSMD14 in pancreatic cancer, we confirmed that the knockdown of PSMD11 and PSMD14 could inhibit the malignant biological behaviors of pancreatic cancer cells, such as their proliferation, migration, and invasion, via the MTT assay, cell scratch assay, and transwell assay, which suggested that these two proteins could be used as potential markers for pancreatic cancer progression and prognostic assessment. This evidence concerns the gene PSMD14 and familial pancreatic carcinoma.