In addition, for the parallel mode of CD28 and 4-1BB, it was demonstrated that dual-targeted CAR-T carrying CD28 and 4-1BB sharing one CD3ζ had higher tumor-killing activity, dividing and proliferating ability and durability than the parallel mode of dual co-stimulatory molecules sharing two CD3ζ (76). The gene discussed is CD247; the disease is neoplasm.