The aim was therefore, to establish an ELISA for the demonstration of IgE autoantibodies to SSA/Ro, SSB/La, RNP proteins and dsDNA in sera from patients with systemic lupus erythematosus (SLE), Sjoegren’s syndrome (SS), and mixed connective tissue disease (MCTD) to investigate their frequency and clinical relevance. Here, RO60 is linked to synovial sarcoma.