GFAP and Alzheimer disease: The levels of p-tau217 and p-tau231 showed the best performance for detecting aMCI /AD from HCs at cut-off values of 4.2 pg/mL and 10.6 pg/mL, achieving AUCs of 0.95 and 0.93, respectively, followed by the levels of p-tau181 and GFAP at cut-off values of 8.7 pg/mL and 24.4 pg/mL, with an AUC of 0.82 and 0.79, and α-synuclein at cut-off value of 3981.6 pg/mL, with an AUC of 0.77.