Nude mice bearing U251 cell line glioma orthotopic xenograft (n = 5) were divided into six groups: (1) Control, (2) Control + CCNU, (3) sh-TRAF7, (4) sh-TRAF7 + CCNU, (5) OE-TRAF7, (6) OE-TRAF7 + CCNU. Here, CCNO is linked to central nervous system cancer.