Additionally, CYTL1 and TLR3 were found to have a significant negative causal relationship with PCOS (CYTL1: IVW: OR = 0.76, 95% CI: 0.62–0.94, P = 1.13e − 2; TLR3: IVW: OR = 0.58, 95% CI: 0.37–0.91, P = 1.73e − 2) (Fig. 3 and Additional file 1: Table S6). The gene discussed is TLR3; the disease is polycystic ovary syndrome.