DEN/CCl4-induced Tfap2aΔHep and Tfap2aΔMΦ mice were found to enhance HCC tumorigenesis by improving DNL synthesis with the activation of SREBP1/ACC/FASN in steatotic hepatocytes and increasing the secretion of inflammatory cytokines in macrophages, respectively. This evidence concerns the gene FASN and hepatocellular carcinoma.