Extensive research has shown that small molecule inhibitors targeting PORCN can effectively block the Wnt signaling pathway, thereby exerting anti-tumor effects.674–678 For example, WNT974 (LGK974) has been demonstrated to reduce the viability of epithelial ovarian cancer (EOC) cells in vitro and to inhibit tumor growth in vivo by blocking Wnt signaling.676 Head and neck squamous cell carcinoma (HNSCC) cell lines harboring inactivating Notch1 mutations exhibit sensitivity to LGK974 inhibition. This evidence concerns the gene NOTCH1 and head and neck squamous cell carcinoma.