Although SOX2 overexpression did not further enhance in vitro migration and invasion in the AKP cancer cell lines (data not shown), SOX2 ectopic expression markedly increased expression of the vimentin and N-cadherin mesenchymal proteins and the EMT-inducing transcription factor TWIST1 in both AKP lines as well as the SNAI2 (also known as Slug) transcription factor in the AKP1 line (Supplemental Figure 9). Here, TWIST1 is linked to cancer.