To test whether DPEP1-expressing CRC cells bind neutrophils, freshly isolated human neutrophils were plated on confluent cultures of SW620 cells, a metastatic CRC cell line reported to have high levels of DPEP1, and its primary tumor counterpart, SW480 cells, which express low levels of DPEP1 (Supplemental Figure 2A) (13, 35). This evidence concerns the gene DPEP1 and colorectal carcinoma.