DPEP1 and neoplasm: To test whether DPEP1-expressing CRC cells bind neutrophils, freshly isolated human neutrophils were plated on confluent cultures of SW620 cells, a metastatic CRC cell line reported to have high levels of DPEP1, and its primary tumor counterpart, SW480 cells, which express low levels of DPEP1 (Supplemental Figure 2A) (13, 35).