To determine whether the enzyme‐activated locus plays a role in the phenotype of BLCA cells, we overexpressed both wild‐type RNF112 (WT‐RNF112) and the inactivation mutant RNF112 (C97A‐RNF112) in BLCA cells (T24 and 5637). Here, RNF112 is linked to bladder transitional cell carcinoma.