To determine whether RNF112 regulates the proliferation, metastasis, and lipid synthesis of BLCA cells via ACLY, we performed plate colony formation assays, transwell migration assays, and triglyceride content measurements after RNF112 and ACLY were individually or simultaneously overexpressed in BLCA cells (T24 and 5637). This evidence concerns the gene RNF112 and bladder transitional cell carcinoma.