In this study, we performed a comprehensive analysis of the interaction between three species of oral bacteria that play distinct roles in periodontitis: the pathobiont Fusobacterium nucleatum, the gram-negative pathogen P. gingivalis, and the gram-positive pathogen F. alocis, and a broad range of inflammatory cytokines in the regulation of COX-2 and PGE2 in GFs. Here, PTGS2 is linked to periodontitis.