•Evaluate expression and localization of SATB1 and SATB2 across paired human colon/rectum and tumor samples•Correlation of expression of SATB1 and SATB2 across different grades and stages of tumor samples towards understanding CRC progression•Understand the role of SATB proteins in tumor regeneration and disease relapse by validation of the association of its expression across differentiated and undifferentiated tumors•Correlation of expression of SATB proteins across colorectal cancer subtypes-Classical adenocarcinoma, mucinous adenocarcinoma, and signet ring pathology tumors. This evidence concerns the gene SATB2 and colorectal cancer.