To test the hypothesis that Riluzole and sodium butyrate combined treatment better decreases aggregation of the h‐SOD1 mutated protein and restores the gut‐brain barrier function, thus delaying the ALS disease onset, we examined ZO‐1 and Claudin‐5 in the cortex of brain tissue. The gene discussed is TJP1; the disease is amyotrophic lateral sclerosis.