As shown in Fig. 9a and b, the tumor site temperatures increased rapidly from 31.5 °C to 54.2 °C (ΔT = 22.7 °C) and 51.8 °C (ΔT = 20.3 °C), respectively, in the CuFeSe2-AMD3100-Gem + laser and CuFeSe2-AMD3100 + laser groups, indicating the efficient photothermal toxicity of CuFeSe2-AMD3100-Gem in vivo. This evidence concerns the gene GEM and neoplasm.