Comparison of DEGs from our VPA-treated hNPCs with known ASD genes from the Simon Foundation Autism Research Initiative (SFARI) (https://gene.sfari.org/), PsychENCODE ASD patient postmortem brains [77], and brain organoids derived from ASD patients [78], revealed extensive overlap and identified five overlapping genes across all four datasets, remarkably similar to the eight overlapping genes in the previously published organoid dataset with chronic VPA exposure, which also included PRKCB, DPP10, and SCN1A (Fig. 2E and S. Table 2). This evidence concerns the gene PRKCB and autism.