As predicted, relative to TXNIP-OE HCC-1954 cells, CAST-KD_TXNIP-OE HCC-1954 cells were significantly more likely to be arrested in the G1 phase (57.92% versus 35.74%, p < 0.0001) and less likely to enter S phase (35.33% versus 51.34%, p < 0.0001) (Fig. 5B; Supplementary Fig. 5G, H). Here, CAST is linked to hepatocellular carcinoma.