Consistent with these in vitro results, CAST-KD MDA-MB-231 tumors in NSG mice exhibited reduced tumor volume and weight relative to WT MDA-MB-231 tumors, whereas CAST-OE MDA-MB-231 tumors displayed greater tumor volume and weight relative to WT MDA-MB-231 tumors (Fig. 3D, E; Supplementary Fig. 3D–G). This evidence concerns the gene CAST and neoplasm.