To this end, we established a mouse GVL model (Supplementary Fig. 11a) by infusing mouse reticulum cell sarcoma A20 cell to explore the GVL effect exerted by the PD-1+CD8+ TSCM-like regulatory cells.67 Briefly, recipients mice were divided into 3 groups and received WT TCD-BM along with 5 × 105 A20 cells; WT TCD-BM, 5 × 105 A20 and WT CD8+CD44−CD62L+ T cells; WT TCD-BM, 5 × 105 A20 and G-CSF-treated WT CD8+CD44−CD62L+ T cells. The gene discussed is CD8A; the disease is reticulum cell sarcoma.