We first detected the protein expression levels of METTL3, METTL14, WTAP, FTO, and ALKBH5 in 112 primary AML samples and found that the percentage of positive cells positive for these 5 enzymes was 80.4% (90/112), 65.5% (73/112), 83% (93/112), 80.4% (90/112), and 58% (65/112), respectively, while no expression was detected in the NCs (n = 16). The gene discussed is METTL3; the disease is acute myeloid leukemia.