Additionally, this patient harbored three additional rare variants in other ALS-associated genes that met our variant filtering criteria: UNC13A p.Leu1034Val, SUSD1 p.Trp704Ser, and SQSTM1 p.His359del, which may have collectively contributed to the development of ALS in this pedigree (Table 2). Here, SQSTM1 is linked to amyotrophic lateral sclerosis.