Additionally, this patient harbored three additional rare variants in other ALS-associated genes that met our variant filtering criteria: UNC13A p.Leu1034Val, SUSD1 p.Trp704Ser, and SQSTM1 p.His359del, which may have collectively contributed to the development of ALS in this pedigree (Table 2). This evidence concerns the gene UNC13A and amyotrophic lateral sclerosis.