In summary, our study identified the CHCHD10 p.Gly66Val variant in Chinese FALS patients for the first time, and revealed its coexistence with three VUS in other ALS-related genes (UNC13A p.Leu1034Val, SUSD1 p.Trp704Ser, and SQSTM1 p.His359del) through whole-genome sequencing. The gene discussed is UNC13A; the disease is amyotrophic lateral sclerosis.